Method For Performing Quantitation Assays



The present invention relates to a method for determining an estimate of a concentration of analyte molecules or particles E(C), wherein a sample of predetermined volume is divided into a number (N) of compartments, the (N) compartments comprise or consist of different sample volumes (Vi) and/or different dilution factors (di) of the sample, at least part of the analyte molecules or particles that are present in any of the (N) compartments provide a measurable signal and the estimated concentration of molecules or particles E(C) is a function of measured signals, as well as an apparatus for use in the inventive method, uses of the inventive method, a sample holder and a kit for use in the inventive method.




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NO-Patent Citations (2)

    COCHRAN W G等: "Estimation of bacterial densities by means of the "most probable number"", 《BIOMETRICS》
    JASON E. KREUTZ等: "Theoretical Design and Analysis of Multivolume Digital Assays with Wide Dynamic Range Validated Experimentally with Microfluidic Digital PCR", 《ANALYTICAL CHEMISTRY》

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