Recombination gene expression system of mortierella alpina and construction method and applications thereof

一种高山被孢霉重组基因表达系统及其构建方法和应用

Abstract

The invention relates to a recombination gene expression system of mortierella alpina (Mortierella alpina ATCC 32222) and applications thereof. According to the invention, by taking mortierella alpina ATCC 32222 uracil auxotroph strains as materials, a set of recombination gene expression system of mortierella alpina is constructed through carrying out genetic manipulation by using an agrobacterium tumefaciens mediated genetic manipulation technique; and an operation is operated by using the system, so that multiple high-yield polyunsaturated fatty acid mortierella alpina strains are obtained, therefore, the recombination gene expression system of mortierella alpina and construction method and applications thereof disclosed by the invention are of great importance in the basic theory study and product development of oil-producing fungi mortierella alpina ATCC 32222.
本发明涉及一种高山被孢霉(Mortierella alpina ATCC 32222)的重组基因表达系统及应用。本发明采用高山被孢霉ATCC 32222尿嘧啶营养缺陷型菌株为材料,运用根癌土壤杆菌介导的遗传操作技术进行基因操作,构建了一套高山被孢霉重组基因表达系统。并运用这套系统操作,得到多株高产多不饱和脂肪酸高山被孢霉菌株,对产油真菌高山被孢霉ATCC 32222的基础理论研究及产品开发具有重要的意义。

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Cited By (6)

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