Method for improving chlamydomonas reinhardtii hydrogen production amount of leghemoglobin ferrous chelate enzyme gene

豆血红蛋白亚铁螯合酶基因提高莱茵衣藻制氢产量的方法

Abstract

本发明涉及生物制氢技术,一种豆血红蛋白亚铁螯合酶基因提高莱茵衣藻制氢产量的方法。现有莱茵衣藻制氢的缺点是:莱茵衣藻氢酶对氧气敏感,容易受氧气的抑制而失去活性;限制了衣藻的产氢效果。本发明公开了一种豆血红蛋白亚铁螯合酶基因和球蛋白亚基基因在莱茵衣藻产氢中的应用,将豆血红蛋白亚铁螯合酶基因hemH和球蛋白亚基基因lba构建在莱茵衣藻叶绿体表达载体中,并将该表达载体转入莱茵衣藻叶绿体中,使hemH-lba基因在莱茵衣藻叶绿体中表达。本发明的优点是:转化的莱茵衣藻的密闭培养体系中氧气含量下降地明显比未转基因莱茵衣藻快,并且氧气含量能维持较低水平,产氢量明显增加。
The invention relates to a biological hydrogen production technology, in particular to a method for improving the chlamydomonas reinhardtii hydrogen production amount of a leghemoglobin ferrous chelate enzyme gene. The traditional chlamydomonas reinhardtii hydrogen production method has the defects that chlamydomonas reinhardtii hydrogen enzymes are sensitive to oxygen and easily restricted by the oxygen to lose activity, and the chlamydomonas reinhardtii hydrogen production effect is limited. The invention discloses an application of a leghemoglobin ferrous chelate enzyme gene and a globulin subunit gene to hydrogen production by constructing the leghemoglobin ferrous chelate enzyme gene hemH and the globulin subunit gene lba in a chlamydomonas reinhardtii chloroplast expression vector, transferring the expression vector in the chlamydomonas reinhardtii chloroplast and expressing the hemH-lba gene in the chlamydomonas reinhardtii chloroplast. The invention has the advantages that the content of oxygen in a closed culture system of the transformed chlamydomonas reinhardtii is reduced obviously faster than that of the chlamydomonas reinhardtii of an un-transformed gene, the oxygen content is kept at a lower level, and the hydrogen production amount is obviously increased.

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Patent Citations (1)

    Publication numberPublication dateAssigneeTitle
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NO-Patent Citations (3)

    Title
    《JOURNAL OF BACTERIOLOG》 19920731 JANA M. FRUSTACI AND MARK R. O"BRIAN Characterization of a Bradyrhizobium japonicum Ferrochelatase Mutant and Isolation of the hemH Gene 第4223-4229页 1 第174卷, 第13期 2
    《Journal of Biotechnology》 20100206 Shuangxiu Wu et al Improved hydrogen production with expression of hemH and lba genes in chloroplast of Chlamydomonas reinhardtii 材料与方法部分,结果部分第3.2节 1 第46卷, 第3期 2
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